By establishing a grouting station and drilling holes in the separation zone for grouting, the grouting in separated-bed can enable the slurry to fill the separation space between the rock layers, limit the movement and deformation of the overlying rock layers and control surface subsidence. This article selects the working face 11-3101 of Mengkeqing Coal Mine in Inner Mongolia as the research background. By studying the rheological characteristics of the grouting filling slurry in the overlying rock separation layer, the material ratio of the filling slurry was determined; A mechanical model for the evolution of mining induced overlying strata separation has been constructed, and a prediction expression for the separation opening has been derived. A method for predicting the dynamic development position of separation has been proposed. The grouting process of overlying rock separation layer was analyzed using UDEC numerical simulation to obtain reasonable grouting pressure and injection production ratio. The technology of grouting in separated-bed was implemented in 11-3101 working face of Gumen Keqing Coal Mine, and surface Deformation monitoring was carried out. The results show that: Four separation layers were generated, respectively located at 45.42m, 228.38m, 337.19m, and 363.99m above the top plate of the working face. The separation opening from bottom to top is respectively 2.62m, 1.49m, 1.39m, and 1.33m. When the grouting pressure is 6.5MPa and the injection production ratio is greater than 45%, the surface inclination deformation value was less than 3mm/m. The monitoring results show that the maximum settlement of the railway roadbed was only 58.72mm, and the grouting has a significant effect on reducing settlement.