Abstract: In order to study the influence of grouting and filling on groundwater quality,the grouting and filling status in No.102 mining area of a mine in Huaibei Mining (Group) Co., Ltd. was used.Based on the study of the spatial relationship between mining fissures and separation zones, the grouting and filling tracing tests were carried out to simulate the grouting and fluid migration laws. The water samples were collected near the grouting station and underground for water quality detection. The results show that the height of caving zone is 12.0-12.8 m, the height of water conducting fracture zone is 46-52.24 m, the effective support area of grouting accounts for 70% of the total mined out area, and the grouting body forms an effective support function; the presence of tracer particles in the lane-way indicates that the grouting fluid has a certain hydraulic relationship with the lane-way; with the passage of time, the grouting fluid migration occurs, and the influence range of migration is within 1 km of the grouting hole. Internally, grouting slurry has no effect on drinking water in Quaternary loose strata, but has a partial effect on the quality of sandstone fissure water in coal strata at the local roof of coal seam. With the dilution of mine water, this part of influence can be neglected.